Analyze bacterial sequence with customized reference

[yu_guoqin]

I have generate bacteria sequence reads not based on 16S or ITS. It is the purpose to target a bacteria genus with the customized the reference database.

How do I analyze it in QIIME2? How do I develop the reference database with the format compatible to the QIIME2?

Thanks!

[Nicholas_Bokulich]

Hi @yu_guoqin,

@yu_guoqin

How do I analyze it in QIIME2? How do I develop the reference database with the format compatible to the QIIME2?

Check out some of the other compatible reference databases here — the raw sequence fasta and taxonomy files are good examples of the expected formats.

I hope that helps!