Analysis workflow

Hi everyone !

My name is Anas, i am currently a trainee and i have been given 16s sequences (fastq.gz) to analyze (they came from healthy vs diseased mussels). Qiime2 seemed to be the best way for me to perform this analysis, so i went through the "moving pictures" and the atacama soil microbiome tutorials with success.

I have very basic knowledge about microbiome analysis but no experience in this topic. Therefore i am asking for help in order to guide me through the workflow of the analysis.

From my sequences (paired end demultiplexed), i would like to generate figures as follows:image


Here is my metadata file, validated with keemei

My purpose is to compare, diversity between healthy and diseased mussels and to point out taxas that may cause the disease.

Thank you in advance for your help !


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