Hello all q2 experts,
Can someone please kindly give me some advice on my situation.
Herewith the summary of my project:
- ITS1F/ITS2 PE300 MiSeq
- demultiplexed input
- dada2 denoise-paired ( --p-trim-left-f 22 --p-trim-left-r 20 --p-trunc-len-f 280 --p-trunc-len-r 180 )
I did try to use ITSxpress plugin and almost everything got filtered out
I am wondering why there is such a big percentage of non-target amplification/ error within all of my samples.