Thanks for your response.
I used both ‘p-rev-comp-barcodes’ and ‘p-rev-comp-mapping-barcodes’ and it worked.
qiime demux emp-paired \
DENOISING PAIR-END READS
I have an issue with the denoising command. I do not want to trim my reads so I used this command:
qiime dada2 denoise-paired
Plugin error from dada2:
An error was encountered while running DADA2 in R (return code -9), please inspect stdout and stderr to learn more.
Debug info has been saved to /tmp/qiime2-q2cli-err-mk4sd79d.log
What am I missing please?