Yes yes, thanks a bunch, I just did that. Another issue is my multiplexing script. I have 8nt nucleotides instead of 12nt. How do I manipulate the command? I used the following command , the error was also attached: qiime demux emp-paired \ --m-barcodes-file sample-metadata.tsv --m-barcodes-co…

Hello @bettya , There are kind of two problems here. First let's address the error: The error is not because you have 8nt vs 12nt ( Although that will be a problem). It is actually causing an error because there is no parameter named --p-golay-error-correction-details. You want either--p-golay-err…

Hi Chloe Thank you for the advice I am unable to demux despite adding the 'no golay error correction'. I attached the error log and I also verbose the command. qiime demux emp-paired \ --m-barcodes-file sample-metadata.tsv --m-barcodes-column BarcodeSequence --p-rev-comp-mapping-barcodes …

Hello again @bettya ! So there problem again described is in the error that you sent: [image] bettya: No sequences were mapped to samples. Check that your barcodes are in the correct orientation (see the rev_comp_barcodes and/or rev_comp_mapping_barcodes options). If barcodes are NOT Golay form…

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Error when running q2-demux

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