18S reference dataset

Hello community,

I am working with an 18S dataset and I am looking for alternatives to the silva one. Is anyone aware of any good 18S reference dataset?

Cheers
Francesco

Hello Francesco,

Silva is the most common and probably the best. But you could also check out PR2 if you are interested in 18S from Eukaryotes.

Colin

Hey @colinbrislawn, is there anyway to use any of these two databases as reference databases in QIIME/QIIME2?

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Of course! Any reference sequence database can be used with QIIME 2, provided you format the database correctly. In fact, the PR2 database was made available in QIIME 2 format a while back by another forum user:

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Thanks everyone, the PR2-R dataset classified many more ASVs at a much deeper taxonomical rank compared to the silva one.

Cheers
Francesco

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