Sorry, let me clarify. But no, RESCRIPt was not implemented until QIIME 2 version 2020.6, and we’ve only provided pre-made files and classifiers for the latest SILVA 138 database since then. But it is perfectly fine to use the pre-made SILVA 132 files as long as you are using the corresponding version of QIIME 2.
We released pre-made SILVA 132 classifiers for 2020.2 and earlier using a different processing approach, with slightly different taxonomy string formatting. That is, we’ve updated the taxonomy prefixes to resemble those of GreenGenes (i.e. p__, c__, etc.) as of 2020.6. So, we only provide pre-made SILVA 138 classifiers and files.
However, you can use the RESCRIPt command
qiime rescript get-silva-data (use the
--help flag to read the help text) to grab version 132 and process / format to your liking, following the tutorial I linked. Note: RESCIPt currently requires QIIME 2 version 2020.8 or later.
Yes, the SILVA database can be used for both 18S and 16S data. Links to the ITS data files are linked in the Data Resources page. You’ll likely have to download and import those yourself.