Hello everyone, try again to import the readings of each of my samples using DADA2, however in the summary of my readings after being filtered and joined, I found that in one of my samples I scored zero number of readings, someone Could you explain to me what was the reason why I got this result?
What happens is that he is reviewing the readings and apparently, that sample (CC_BC) contains adapters that were not removed by the company with which he ordered to sequence, I do not know if that is what is influencing
That definitely explains why it looks different from the others, although the 0 is still a bit strange. Do you have the DADA2 output stats generated alongside this feature-table? It would explain where the reads disappeared. Alternatively, it's possible demultiplexing failed further upstream if the adapters are still present, which would explain the zeros I think.
Either way, good thing you caught this and glad to hear it's being worked out!