Hi team,
I have downloaded data from ncbi. The link to the project is given SRP002457 - SRA - NCBI
when I download data from each experiment I get a 'sra.data.fastq' file and "SraRunTable.txt" file corresponding to each experiment.
the spot descriptor in the ncbi page is shown like this
my reads are like this when I did 'head sra_data.fastq'
@SRR089478.1.4 GJL9MXC01BO4HY length=176
ACATTACCGCGGCTGCTGGAGTAGGCAAGTTAGGCTCTAATGCCTTCACAAGTTCCATAGCCTCATCAACCCTTCACACAACCTGATCTGTCCTCCTAATTCCAAATATGTGTGAGTTAGAGTCCTGAGCCAGGATCAAACTCTCTGAGACTGCCAAGGCACACAGGGGATAGGNN
+SRR089478.1.4 GJL9MXC01BO4HY length=176
IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIICC???IIIIIIIIIIIIIIIIIIIIIIIIIIIIIII???IIIIIIIIIIIIIIIIHCCDIDCCCEEIICCCCCIIIIIEGGEEE??9;;;;;IIIIII@@BBIIIC?!!
@SRR089478.2.4 GJL9MXC01A3F14 length=522
GACATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGACGTCAACGGTNTAACTTAACGCCTTCCTCCCCGATCGAAAGTCTTTACAACCGAGGCCTTCTCATCACGCGGCATCGGCTGCATCACGCTGCGTCCATTCGTGCAATNTCCCATGCCTAGCCTCCGTAGGCTCGNGGACCTGCCTAGCGTCCATGGNTCGTGCCTGTCGCTCAGCATGCGGATCGCAACCTGTGCGCCTTACCCAGTCAGGCCGTAAATCCATCGTGGNACANCCGATGCAGCGCCAGGCCCTATGCTNCGCTTNGACTATTNCCAGTTCAATCCCTTCCCGAGNGTCGCGTCNGTTCCATCCGCGTTCCCNCTACGTACGACTCGTACGTCTCGTACGAACTCGCTACGTCCGTACGTTCGTACGTACGTTCGCTTACGGTCGTACGTCGTACGTCCGTCGTACGCCGTCGTACGTCGTTACGTCGTACCGTACGTCGACCTAACGTCG
+SRR089478.2.4 GJL9MXC01A3F14 length=522
1:ABBA=::<HHHIHIIIIIIIIBBBHHFDBBA88984--/112,//,,,/59=35211777////--/--/!/--4--,,-.----,,,,,,,..3371,,-..,133-----.,--991-,,-33439:212344/..=::733443///47==557557:55311.!-,,,.331-//49=>><===2244//!--3117.--3:::8=1133--!...5891..-.--.233333..-13...-151-------11117111----35711,-,,,975-------!--5!55733373331978--,,,371333!3----!3----55!1187553---,,,-5,,,,3,!,,,,0430!305663322...,4110!-,,,,,,,,,,30,,,,,0000754000003,,,,,,,..34441,,,,,,01,,,,,,,,,,,,,,,,,,,,04411,,,,,,,,,,,,.0.,,,10.,,,,,,,,,,0,,,,,,,,..,,,,,,,,,,,,,,,,.1
@SRR089478.3.4 GJL9MXC01A54GP length=147
CTGGTCCTTGTCCTTTGCAAATTTATTCGTAACAGGCGAGGTGTAGGGAGGCAGATACTTCCCGCGGTCGCTCAGTGAAATCTTAGAGCACAGCTCCTGAGCCAGGATCAAACTCTCTGAGACTGCCAAGGCACACAGGGGATAGGN
I wanted to do analysis with QIIME
But how will I get barcode as afile from ncbi if the data is multiplexed. pls help