Hi. I’m working on a dataset including microbiome data and clinical data.
I want to analysis difference of abundance across clinical parameters,(disease, sex, age…)
As far as i know, LEfSe does 2 statistical test, of which one uses class and the other use subclass.
My question is… when a dataset have no subclasses to compare, could i use LEfSe algorithm?
My colleague showed a ancom analysis result on the same dataset and i found the lefse result show me much more otus than the ancom analysis…