What is the difference between a read, sequence, and ASV?

I am currently writing a manuscript and am very confused when to use each of these terms. Currently, my understanding is that I have many reads over the 16rRNA region. These reads are then lined up against a reference genome and then are used to find a "consensus sequence". From there, demultiplexing and trimming my sequences results in amplicon sequence variants, which are the unique taxonomic features present in my sequences.

1 Like

Hello Emily,

Welcome to the forums! :qiime2:

You may be interested in the conceptual overview of Qiime2. This covers exactly how sequencing reads end up in a feature table of ASVs. That page also provides good examples how how these terms are used.

Take a look at those graphs and post your follow-up questions here!


Will do, thank you!!