I have a similar issue. Can I jump in here please or should it be a separate query? I have got sequences from a MiSeq 2x300bp V4 run. there are 12 human fecal samples and I got 2,567 features. I have not got I am using QIIME2, 2019.7. I have attached here the paired-end-demux.qzv Paired_end_demux.qzv (290.6 KB) .
Then I did
qiime dada2 denoise-paired
I have attached the stats-dada2.qzv stats-dada2.qzv (1.2 MB) .
I am now trying with primer cut offs of 20 (which was advised in another post), left truncation at 280, and right at 270, to check the difference.
So my questions are aren’t the number of features too high i.e. the dada2cutoff not stringent enough? Am I on the right track?
Also attached here is the taxonomy0.7qzv taxonomy_gg0.7.qzv (1.5 MB) , if you have any comments please.
Thanks very much.