there are something mysterious happened in my qiime2：
qiime vsearch cluster-features-closed-reference
when i run code above with 85_otus.qza from official (https://data.qiime2.org/2019.10/tutorials/otu-clustering/85_otus.qza), everything is okay, while when i run the same code with the same qza imported from 85_otus.fastq, there is a error occurred. and this is the tips:
Plugin error from vsearch:
No matches were identified to reference_sequences. This can happen if sequences are not homologous to reference_sequences, or if sequences are not in the same orientation as reference_sequences (i.e., if sequences are reverse complemented with respect to reference sequences). Sequence orientation can be adjusted with the strand parameter.
Debug info has been saved to /tmp/qiime2-q2cli-err-hdo1wth7.log
and 85_otus.qza is the file downloaded from official web.
86_otus.qza is the file i import use the code below.
the picture is the differece when i view it in command line, the official 85_otus.qza is green! and other qza i import such as 85,97,99 are all white and all invalid.
qiime tools import