Dear-Bod and qiime 2 developers
I would like to pre-train classifier of V3-V4 (341F, 805R) region with gg_99. I have the paired-end 16S seq with 300 base-paired read generated by MiSeq with trimmed forward length 270-290 basedand trimmed reverse length 210-230 base. Here is the commend line I’m going to use. I’m not sure what the number I should put with a question mark below:
qiime feature-classifier extract-reads
–p-trunc-len 300 (?)
–p-min-length 200 (?)
–p-max-length 550 (?)
Your help will be greatly appreciated. I went to the Qiime 2 workshop and didn’t get a help for this specific question.
Thank you very much in advance!