using LEfse on the galaxy

Hello everyone.
sorry if my question is not related to qiime. It’s my first time that I want to use galaxy specifically LEfse.
I have read the tutorial and it seems it’s easy to use but I think there is a problem which I don’t know what is it.
I exported my data from qiime2 and make it in a form of relative abundance and “biom with taxonomy.tsv” and then kept just genus level. if any row didn’t have genus level I used the higher taxa level but with () around the name.
but when I use LEfse the result doesn’t make sense. could you please let me know why?
I send you my data and the result picture.


Thank you very much

hi Mohsen,

Since I use QIIME data for LEFSE, I think we can add class and subclass properly before we get LEFSE plot. May you try to do it first before you plot your LEFSE result

Hope it helps.

thank you. but my data doesn’t have any subclass or something because its just about some kinds of meats.