Hei everyone, i have a trouble when trying Atacama soil microbiome tutorial, the problem is like in the picture, am i do something wrong? by the way, iam using 2018.2 versionScreenshot%20from%202018-05-14%2009-26-58|690x336
Could you provide the contents of the error log file mentioned or if it is gone already just re-run the command with the '--verbose' flag included.
Another point, though I don't think this is related to your current error message, but the tutorial has a truncating parameters of 150 and trimming of 13, in your commands you have truncating of 130 and trimming of 15. I don't know enough about this particular dataset's overlap region to guess if this is going to cause overlapping issues during merging of dada2, but if you are following this tutorial all the way through, consider that you might have some different outputs because of this seemingly small change.
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@Mehrbod_Estaki iam already following the tutorial, but it still the same
That's good that you fixed the truncating parameters but we still need the content of the error message to help with the troubleshooting. Could you please re-run your command with the --verbose tag added and report the output.
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