I'm wondering if it is possible to add an option to trim from the 5' when using Deblur. Currently there is only a trim-length option but in some instances a few very low quality bases initially appear in MiSeq runs (10-15 bp) that would probably be better trimmed. This would be like DADA2's trunc_len_f
option.
See the link provided in this post for an example of low quality reads on the left.
Hi @Mehrbod_Estaki! That is a great idea - maybe @wasade (the maintainer of q2-deblur
) can provide some additional comments. Thanks!
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Thanks, @Mehrbod_Estaki! Sorry for the delay, I did not see this until now. @yoshiki coincidentally opened an issue about this yesterday, and the changes for Deblur
are currently in review. We have not issued the necessary revisions yet to q2-deblur
as we'll need to cut a micro release of Deblur
first.
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No problem @wasade and glad someone else was thinking the same thing as well!
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