Hello, I´m Vicky again!
A few days ago I received my .fastq.gz files from the V3-V4 region, the sequencing was done in a NextSeq. The demultiplexing step took about 2 hrs, and we decided to cut at 240 bp to do DADA2, it has been running for more than 48 hrs and does not finish yet.
I have 56 samples and the input file of DADA2 has 11.1G, do you think that by the gigabytes, quality and number of samples it takes much longer? I’m working on a cluster or server, which has more memory than a local computer.
I am not surprised at all that dataset 11 Gb in size is taking more than 2 days to be complete - this size means that there are a lot of sequences to process.
What surprise me is that you have only 56 samples - usually my samples from NextSeq and in the same number are much lighter (1-2 gb or less).
Are you think there’s a mistake in the FASTQ documents that loaded in my BaseSpace?
No, I just surprised by the size of the library, but it is not necessarily an error.
This topic was automatically closed 31 days after the last reply. New replies are no longer allowed.