Thanks a lot!
I was able to make an amplicon specific classifier for V1-V3 region with primers 27f and 515r.
I evaluated the classifier against the 85_OTU.qza provided, and got 100% classification accuracy.
Should I test it against a more complex dataset like Greengenes 97% OTUs?
I also would like to know if I can use the same ref taxonomy provided in this tutorial, or one specific for the greengenes 97.
Lastly, I am new to this world, and I don’t know where to the those reference sequences from. Could you point me where to get them from?
thanks a lot to you and to this wonderful community!