Hello Fedarko,
I have a couple of questions related with mentioned discussion. (1) Is it appropriate to use multivariate option in songbird to analyze a single factor data? Like I want to analyze the factor "Treatments" that has Six sub-levels and seek differential microbiota in five levels in comparison with Control group (Reference). (2) When songbird differentials is used as input in Qurro it generates Natural_Log_Ratios (after selecting reference frame) for features across different groups. Should we use the output data of Natural_Log_Ratios for statistical analysis (ANOVA or Kruskal-Wallis etc with post hoc tests) to see significant differences among groups?
I would be very thankful for your comments/answers.
Best regards,
Bilal