Hello I am using Qiime2-2022.11
I am getting a error in the taxaplot step:
This are the commands :
qiime taxa barplot
--i-table table_.qza
--i-taxonomy taxonomy_.qza
--m-metadata-file Metadatos_.tsv
--o-visualization taxa-bar-plots_.qzv
Plugin error from taxa:
Feature IDs found in the table are missing from the taxonomy: {'e471b4d90051213a2f380260fc40378f', '6ae397141328fcc917b9a87aa5d548a3', '4c5b12e3825577cde8437df04f5536cc', 'c6d453157c99855d562c971b88788b3c', '6f61d8be1f0a019dc7e7a7ae07c54d08', '5169c226a333dcb12102eff6cd3ad818', '7fee385ad9598137793ac77c7e7c11b4', '043761376a7b4bbf5ed08e472972dd4f', '8ea54389c04fc34bcf3487220ad73af0', '950a9b08dd59529ce33fc4c92c45165b', 'c9a6fea22a7d3e590311fa5674ad44db', '1c7f2d2baa0b3c0e45c3423b4d62d091', 'b3b31167d7d79314b45e782a7939b17e', 'a70c70e204e3b5a7632b047988b6b1ef', 'a1d62669ba5f67dd079c2f7ff250f1fe', 'f66631894ca0b6bbfb15c71c0c6fde10', ' etc. etc.
Thank you
I will try it.
He uses Qiime2-2023 , and I am using 2-2022.11, would that be an issue?
I noticed several different steps in the pipeline, starting on step 7 : qiime feature-table filter-samples
Thanks
The version of qiime will almost certainly not be an issue. The post I linked wasn't meant to be a pipeline to follow, but rather contain an explanation of why/how your feature table and taxonomy became out of sync, which is the root of the issue here.
You're classifying the reference-sequences in step 10. Although this will result in a very accurate classification , this isn't what you want. You want to classify your sequences, namely rep-seqs.qza, which came out of dada2.