short sequences leaking through cutadapt

Hi. This is the cutadapt command I ran:-
qiime cutadapt trim-single --i-demultiplexed-sequences demux_seqs_v2.qza --p-cores 8 --p-minimum-length 400 --o-trimmed-sequences demux_seqs.qza --p-adapter CCTAYGGGDBGCWGCAG...ATTAGAWACCCBNGTAGTCCGG --p-discard-untrimmed --p-match-adapter-wildcards

I do use a length filter of 400 bases. But when I see my representative sequences, there are sequences that are just 22 bases in length. How is this possible? How are those short sequences not being removed? Can someone please help.

Hi @ankurnaqib,
That seems like a strange behavior indeed, would you mind sharing with us your trimmed-sequences demux_seqs.qza file so we can take a closer look? You can DM that file if you have concerns about sharing it publicly.

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