Sample ID in feature table and meta data not in order

[Parikrama_Sapkota]

A. I got this error (below) for this code

physeq <- qza_to_phyloseq(

• features = "/Users/parikramasapkota/Desktop/CedricWind/durp_unpooled_rhizosphere/dada2_table_16S.qza",
• tree = "/Users/parikramasapkota/Desktop/CedricWind/durp_unpooled_rhizosphere/phylogeny-align-to-tree-mafft-fasttree/rooted_tree.qza",
• taxonomy = "/Users/parikramasapkota/Desktop/CedricWind/durp_unpooled_rhizosphere/taxonomy.qza",
• metadata = "/Users/parikramasapkota/Desktop/CedricWind/durp_unpooled_rhizosphere/metadata16S.tsv"
• )
  Error in validObject(.Object) : invalid class “phyloseq” object:
  Component sample names do not match.
  Try sample_names()

As I was trying to look into detail, this was the error, I didn't know how I got "sa" infront of my sample ID

[colinbrislawn (Colin J Brislawn)]

Hello Parikrama,

Phyloseq does not support SampleIDs that start with numbers, so during import it adds this prefix. (GitHub issue.)

This is something Phyloseq does automatically (and without telling you )

The workaround is to rename your samples so they match.

[Parikrama_Sapkota]

thank you! I will try doing that.