Request for Pre-trained UNITE Classifier (ITS1)

Hi everyone,

I'm following the ITSxpress tutorial (Q2-ITSxpress: A tutorial on a QIIME 2 plugin to trim ITS sequences) and trying to use the classifier.qza file mentioned there as a pre-trained classifier. However, when I try to download it from the GitHub repository, I only get a Git LFS pointer file, and the error says that "Git LFS is disabled for this repository", so I can't access the actual file.

I also tried downloading one of the official classifiers from data.qiime2.org, but the link I found returns a 404 error.

Could someone please share an updated or alternative link to a pre-trained classifier based on UNITE (preferably for ITS1), compatible with QIIME 2 version 2021.4, 2024.2 (or similar)?

Thank you very much in advance.

Best regards,
mleon

Hi there!
You can download the file from given link. A ready-to-use ITS classifier for fungal community analysis in QIIME 2, is available in the GitHub repository. The file is tracked using Git LFS, so please make sure to have Git LFS installed before cloning or downloading. Instructions can be found in the README file. Thanks

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Welcome to the forums, mleon! :qiime2:

I've made some of these here for different versions of Qiime2
GitHub - colinbrislawn/unite-train: 🍄 Qiime2 ITS classifiers for the UNITE database

So... we only officially support the most recent release of Qiime2. It's okay to keep using older versions, as the existing methods they implement are still valid, but my advice is going to start with trying to update. I know that can take time, so let's start now!

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Hi @Szahra and @colinbrislawn,

Thank you both so much for your quick and helpful replies!

I installed Git LFS as suggested and was able to successfully download the pre-trained classifier from the repository. I really appreciate the guidance and the work you’ve done to make these resources available—it's a huge help for those of us working with ITS data in QIIME 2.

Thanks again for your support and for keeping the community resources up to date!

Best, mleon

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