My forward and reverse sequences have min and max length of 80 and 150 respectively.
after importing them into qiime2 , I used dada2 plugin for filtering, merging and feature table construction.
When I checked rep-seqs.qzv file, it showed minimum sequence length 80. This is quite unexpected as a minimum length of the reads itself was 80. Are they really merged sequences??
And even if I see them as technical replicates, they should be very few in numbers. So if I filter these sequences on the basis of frequency, they should get eliminated. but I can still see them in my filtered-rep-seqs.qzv.
Is there any way to remove low length sequences? or any other suggestion?
Thanks in advance