RE: PICRUSt2 reference KEGG Database

can you help, please by the exact workflow-commands to run q2-PICRUSt2 for fungal ITS data?
Thank you.

Hi there,

The q2-picrust2 plugin is just for the default 16S pipeline. You can run ITS with the standalone version (see:, but I don’t recommend this for standard analyses because the predictions are very low accuracy.



1 Like

Thank you for your reply :slight_smile:

Hi @nazarGL,
Though not really the same thing as what PICRUTS2 does for 16S predictions, you may be interested in checking out the FUNguild tool/paper if you are trying to extract functional information from your fungi data. Note I have never used it myself, just passing on info.

1 Like