RE: PICRUSt2 reference KEGG Database


Hi
can you help, please by the exact workflow-commands to run q2-PICRUSt2 for fungal ITS data?
Thank you.

Hi there,

The q2-picrust2 plugin is just for the default 16S pipeline. You can run ITS with the standalone version (see: https://github.com/picrust/picrust2/wiki/Frequently-Asked-Questions#how-can-i-run-a-custom-or-non-default-database-such-as-the-fungi-18s-and-its-databases), but I don’t recommend this for standard analyses because the predictions are very low accuracy.

Best,

Gavin

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Thank you for your reply :slight_smile:

Hi @nazarGL,
Though not really the same thing as what PICRUTS2 does for 16S predictions, you may be interested in checking out the FUNguild tool/paper if you are trying to extract functional information from your fungi data. Note I have never used it myself, just passing on info.

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