Thanks. After running for 5 days, the above-mentioned script has failed due to the timeout. Then, I read " A DADA2 workflow for Big Data (1.4 or later)", and I don’t understand how to use the final output file (.rds) to continue anlysis. Should I use Deblur instead of Dada2? But Deblur looks more slow than Dada2 according to the forum.I am stucked in the problem for a long time. Hope to your reply, Thanks again.
Hi @terren - I have recategorized this post to “Other Bioinformatics Tools” since you have moved out of QIIME 2 and into DADA2, we won’t be able to provide direct help.