For this study (as many in Qiita), the raw data is not available ( sequences, barcodes, and metadata). So, if you want to apply the ‘Moving Pictures’ tutorial for this case, you should modify some steps at the beginning of this tutorial (at less until demultiplexed files) and download other files in Qiita.
After clicking in the link that you left above and enter in the study description, follow the steps in the pic:
Once you’ve downloaded the file “741_seqs.fastq” open it and see the structure, you’re going to see that barcode sequences are inside the sequences. So the next step is make a demultiplex of the samples with the sequences but before of this, compress this raw file with gzip and change its name, after this, you’ll get some like this forward.fastq.gz
Now, import this raw file in a Qiime2 artifact, using the type “MultiplexedSingleEndBarcodeInSequence”. Your instruction should look like this:
qiime tools import
Download the metadata from Qiita in the section “Sample information” and format it following the recommendations of Qiime2 developers. Or see the example that they give here https://docs.google.com/spreadsheets/d/15dH-TIA0td8qcC-ali0g2snXG8Dl26wGMKz1Zcji6kA/edit#gid=0
Now, make demultiplexing of your raw file, using the artifact created previously and the formatted metadata with the plugin “cutadapt” and the method “demux-single: Demultiplex single-end sequence data with barcodes in-sequence”. Your instruction, should look like this:
qiime cutadapt demux-single
If you want see more details about this, see this post: Demultiplexing and Trimming Adapters from Reads with q2-cutadapt
Once finished the process, see inside output artifact and you’ll see your demultiplexed samples, like this:
After this, I guess that you can follow the “moving pictures tutorial normally” at the step " Sequence quality control and feature table construction".
Hope that this can help you