Qiime2 Timezone error

Hi all,
I am having a problem with the timezone setting while running Qiime2Picrust. Previously, while running the tutorial I fixed this problem in this way and it was fixed. But this problem is recurring again!

My Commands:
1. Starting:
(base) msiddiq7@enggpz1p23:~ export TZ=‘Eastern/Newyork’ (base) msiddiq7@enggpz1p23:~ conda activate qiime2-2019.10
(qiime2-2019.10) msiddiq7@enggpz1p23:~$ conda install q2-picrust2=2019.10 -c conda-forge -c bioconda -c gavinmdouglas
Collecting package metadata (current_repodata.json): done
Solving environment: done

==> WARNING: A newer version of conda exists. <==
current version: 4.8.3
latest version: 4.9.0

Please update conda by running

$ conda update -n base conda

All requested packages already installed.

2. Command exxecution:
(qiime2-2019.10) msiddiq7@enggpz1p23:~/Kang_dataset_picrust$ qiime fragment-insertion sepp --i-representative-sequences rep-seqs.qza --p-threads 1 --i-reference-database picrust2_default_sepp_ref.qza --output-dir kangdata_out
Plugin error from fragment-insertion:

Timezone offset does not match system offset: -18000 != 0. Please, check your config files.

Debug info has been saved to /tmp/qiime2-q2cli-err-482kp7rs.log
(qiime2-2019.10) msiddiq7@enggpz1p23:~/Kang_dataset_picrust$

#qiime2-q2cli-err-482kp7rs.log.txt (8.4 KB)

I have an additional question:
While running the command for data analysis, I have used the picrust2_default_sepp_ref.qza file which I have downloaded during running the tutorial dataset. I am not sure if I need to use other files.

(qiime2-2019.10) msiddiq7@enggpz1p23:~/Kang_dataset_picrust$ qiime fragment-insertion sepp --i-representative-sequences rep-seqs.qza --p-threads 1 --i-reference-database picrust2_default_sepp_ref.qza --output-dir kangdata_out

Thanks in advance.

Hi @Mashuk!

You have two options here: you can either fix up your system's timezone settings (see [Pinned] ValueError: Timezone offset does not match system offset · Issue #79 · regebro/tzlocal · GitHub for details), or upgrade your version of QIIME 2 (to at least 2020.2, where we added in a few guards to handle this).

Please ask this in a new topic (in "Community Plugins") - one question per topic thread is best.

Thanks!

:qiime2:

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