Hello - I was using qiime2-2018.6 got to the phylogeny section, stalled, found this link for why…
align to tree mafft fasttree missing
Proceeded to install 2018.8 and got the following…
(qiime2-2018.6) wsb255bioimac27:Issue_0033_cutadapt_qiime2 mel_local$ source deactivate qiime2-2018.6
wsb255bioimac27:Issue_0033_cutadapt_qiime2 mel_local$ conda update conda
Fetching package metadata ...............
Solving package specifications: .
Package plan for installation in environment /Users/mel_local/miniconda2:
The following NEW packages will be INSTALLED:
futures: 3.2.0-py27_0 conda-forge
The following packages will be UPDATED:
conda: 4.3.30-py27h407ed3a_0 --> 4.5.11-py27_0 conda-forge
pycosat: 0.6.2-py27h085d4cc_0 --> 0.6.3-py27h470a237_1 conda-forge
Proceed ([y]/n)? y
futures-3.2.0- 100% |######################################################################################################################################| Time: 0:00:00 750.07 kB/s
pycosat-0.6.3- 100% |######################################################################################################################################| Time: 0:00:00 1.67 MB/s
conda-4.5.11-p 100% |######################################################################################################################################| Time: 0:00:00 2.16 MB/s
wsb255bioimac27:Issue_0033_cutadapt_qiime2 mel_local$ wget https://data.qiime2.org/distro/core/qiime2-2018.8-py35-linux-conda.yml
--2018-09-24 09:44:20-- https://data.qiime2.org/distro/core/qiime2-2018.8-py35-linux-conda.yml
Resolving data.qiime2.org (data.qiime2.org)... 104.27.171.158, 104.27.170.158, 2400:cb00:2048:1::681b:ab9e, ...
Connecting to data.qiime2.org (data.qiime2.org)|104.27.171.158|:443... connected.
ERROR: cannot verify data.qiime2.org's certificate, issued by ‘CN=COMODO ECC Domain Validation Secure Server CA 2,O=COMODO CA Limited,L=Salford,ST=Greater Manchester,C=GB’:
Unable to locally verify the issuer's authority.
To connect to data.qiime2.org insecurely, use `--no-check-certificate'.
wsb255bioimac27:Issue_0033_cutadapt_qiime2 mel_local$ wget --no-check-certificate https://data.qiime2.org/distro/core/qiime2-2018.8-py35-linux-conda.yml
--2018-09-24 09:44:35-- https://data.qiime2.org/distro/core/qiime2-2018.8-py35-linux-conda.yml
Resolving data.qiime2.org (data.qiime2.org)... 104.27.171.158, 104.27.170.158, 2400:cb00:2048:1::681b:ab9e, ...
Connecting to data.qiime2.org (data.qiime2.org)|104.27.171.158|:443... connected.
WARNING: cannot verify data.qiime2.org's certificate, issued by ‘CN=COMODO ECC Domain Validation Secure Server CA 2,O=COMODO CA Limited,L=Salford,ST=Greater Manchester,C=GB’:
Unable to locally verify the issuer's authority.
HTTP request sent, awaiting response... 302 FOUND
Location: https://raw.githubusercontent.com/qiime2/environment-files/master/2018.8/release/qiime2-2018.8-py35-linux-conda.yml [following]
--2018-09-24 09:44:36-- https://raw.githubusercontent.com/qiime2/environment-files/master/2018.8/release/qiime2-2018.8-py35-linux-conda.yml
Resolving raw.githubusercontent.com (raw.githubusercontent.com)... 151.101.128.133, 151.101.192.133, 151.101.0.133, ...
Connecting to raw.githubusercontent.com (raw.githubusercontent.com)|151.101.128.133|:443... connected.
WARNING: cannot verify raw.githubusercontent.com's certificate, issued by ‘CN=DigiCert SHA2 High Assurance Server CA,OU=www.digicert.com,O=DigiCert Inc,C=US’:
Unable to locally verify the issuer's authority.
HTTP request sent, awaiting response... 200 OK
Length: 4998 (4.9K) [text/plain]
Saving to: ‘qiime2-2018.8-py35-linux-conda.yml’
qiime2-2018.8-py35-linux-conda.yml 100%[===============================================================================================>] 4.88K --.-KB/s in 0s
2018-09-24 09:44:36 (33.1 MB/s) - ‘qiime2-2018.8-py35-linux-conda.yml’ saved [4998/4998]
wsb255bioimac27:Issue_0033_cutadapt_qiime2 mel_local$ conda env create -n qiime2-2018.8 --file qiime2-2018.8-py35-linux-conda.yml
Solving environment: failed
ResolvePackageNotFound:
- gstreamer=1.12.5
- gst-plugins-base=1.12.5
- libgfortran-ng=7.2.0
- libgcc-ng=7.2.0
- libgcc=7.2.0
- libstdcxx-ng=7.2.0
Advice?
Do I need to find/install the above packages?
