I tried to do the first step for the deblur - quality-filter:
qiime quality-filter q-score --i-demux ok70_demux.qza --o-filtered-sequences demux-filtered.qza --o-filter-stats demux-filter-stats.qza
and i got this error message:
Plugin error from quality-filter:
Argument to parameter ‘demux’ is not a subtype of SampleData[SequencesWithQuality].
Debug info has been saved to /tmp/qiime2-q2cli-err-3q4h9b_g.log
what an I doing wrong…?
Do you know how you created that qza? Are you sure it’s a
SampleData[SequencesWithQuality]? If you are not sure you can check via
qiime tools peek ok70_demux.qza.
I tried to do it on [PairedEndSequencesWithQuality] :-\
Hi @Adi, can you please provide the full output when running the
peek command suggested above? Thanks!
Data format: SingleLanePerSamplePairedEndFastqDirFmt
The issue here is
q2-deblur cannot use paired-end data unless it has already been merged/joined. If you look at our pending release notes for the upcoming 2017.11 release, you’ll see that we’re adding support for joining your paired-end data. So you should have some options available soon!
The QIIME 2 2017.11 release has expanded support for analyzing paired end reads! See the paired end reads community tutorial for more details.
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