Warning: [blastn] Examining 5 or more matches is recommended
/home/lucy2/anaconda3/envs/qiime2-2023.7/lib/python3.8/site-packages/q2_feature_classifier/_blast.py:80: FutureWarning: The frame.append method is deprecated and will be removed from pandas in a future version. Use pandas.concat instead.
missed = missed.append(
/home/lucy2/anaconda3/envs/qiime2-2023.7/lib/python3.8/site-packages/q2_feature_classifier/_blast.py:84: FutureWarning: The frame.append method is deprecated and will be removed from pandas in a future version. Use pandas.concat instead.
result = result.append(missed, ignore_index=True)
Running external command line application. This may print messages to stdout and/or stderr.
The command being run is below. This command cannot be manually re-run as it will depend on temporary files that no longer exist.
Command: blastn -query /tmp/qiime2/lucy2/data/a13b666f-c976-48bb-840f-137bf7b6e91b/data/dna-sequences.fasta -evalue 0.001 -strand both -outfmt 6 -subject /tmp/qiime2/lucy2/data/b74b3017-b466-41a3-9078-f263af4f6f7f/data/dna-sequences.fasta -perc_identity 80.0 -qcov_hsp_perc 80.0 -max_target_seqs 1 -out /tmp/q2-BLAST6Format-rj0z94e8
Traceback (most recent call last):
File "/home/lucy2/anaconda3/envs/qiime2-2023.7/lib/python3.8/site-packages/q2cli/commands.py", line 478, in call
results = self._execute_action(
File "/home/lucy2/anaconda3/envs/qiime2-2023.7/lib/python3.8/site-packages/q2cli/commands.py", line 544, in _execute_action
results = action(**arguments)
File "", line 2, in classify_consensus_blast
File "/home/lucy2/anaconda3/envs/qiime2-2023.7/lib/python3.8/site-packages/qiime2/sdk/action.py", line 342, in bound_callable
outputs = self.callable_executor(
File "/home/lucy2/anaconda3/envs/qiime2-2023.7/lib/python3.8/site-packages/qiime2/sdk/action.py", line 647, in callable_executor
outputs = self._callable(scope.ctx, **view_args)
File "/home/lucy2/anaconda3/envs/qiime2-2023.7/lib/python3.8/site-packages/q2_feature_classifier/_blast.py", line 102, in classify_consensus_blast
result, = search_db(query=query, reference_reads=reference_reads,
File "/home/lucy2/anaconda3/envs/qiime2-2023.7/lib/python3.8/site-packages/qiime2/sdk/context.py", line 140, in deferred_action
return action_obj._bind(
File "", line 2, in blast
File "/home/lucy2/anaconda3/envs/qiime2-2023.7/lib/python3.8/site-packages/qiime2/sdk/action.py", line 342, in bound_callable
outputs = self.callable_executor(
File "/home/lucy2/anaconda3/envs/qiime2-2023.7/lib/python3.8/site-packages/qiime2/sdk/action.py", line 583, in callable_executor
self.signature.coerce_given_outputs(output_views, output_types,
File "/home/lucy2/anaconda3/envs/qiime2-2023.7/lib/python3.8/site-packages/qiime2/core/type/signature.py", line 487, in coerce_given_outputs
output = self._create_output_artifact(
File "/home/lucy2/anaconda3/envs/qiime2-2023.7/lib/python3.8/site-packages/qiime2/core/type/signature.py", line 511, in _create_output_artifact
artifact = qiime2.sdk.Artifact._from_view(
File "/home/lucy2/anaconda3/envs/qiime2-2023.7/lib/python3.8/site-packages/qiime2/sdk/result.py", line 362, in _from_view
artifact._archiver = archive.Archiver.from_data(
File "/home/lucy2/anaconda3/envs/qiime2-2023.7/lib/python3.8/site-packages/qiime2/core/archive/archiver.py", line 412, in from_data
process_alias, data_path = cache._rename_to_data(uuid, path)
File "/home/lucy2/anaconda3/envs/qiime2-2023.7/lib/python3.8/site-packages/qiime2/core/cache.py", line 1088, in _rename_to_data
process_alias = self._alias(uuid)
File "/home/lucy2/anaconda3/envs/qiime2-2023.7/lib/python3.8/site-packages/qiime2/core/cache.py", line 1116, in _alias
self.named_pool._make_symlink(uuid, uuid)
File "/home/lucy2/anaconda3/envs/qiime2-2023.7/lib/python3.8/site-packages/qiime2/core/cache.py", line 1514, in _make_symlink
os.symlink(src, dest)
FileNotFoundError: [Errno 2] No such file or directory: '/tmp/qiime2/lucy2/data/315d82bf-9c5f-4f61-85b2-de0ef8035fd6' -> '/tmp/qiime2/lucy2/pools/recycle_feature_classifier_classify_consensus_blast_cc604076b532c79727f9cc558b0ab11e64eaf942/315d82bf-9c5f-4f61-85b2-de0ef8035fd6'