I am running Qiime 2 version 2019.1.0.
I have been working with Illumina R1, R2, I1 and I2 files as my input data. I have reviewed this post
https://forum.qiime2.org/t/dmx-with-4-initial-read-files-i1-i2-r1-r2/4190/3 and have performed the following:
Merged the I1 and I2 index files into one by simply concatenating the sequence and quality lines. For example, using this I1 read and I2 read:
@M70271:95:000000000-C7WK3:1:1101:11188:1742 1:N:0:1 TCGACGTC + -,,,8+8, @M70271:95:000000000-C7WK3:1:1101:11188:1742 2:N:0:1 TCTTCTTT + -,,,,6,6
I create this merged read:
@M70271:95:000000000-C7WK3:1:1101:11188:1742 TCGACGTCTCTTCTTT + -,,,8+8,-,,,,6,6
I removed all primers from my R1 and R2 reads using cutadapt.
I created a metadata file and verified that it is properly formatted using keemei. My metadata file looks like this:
I then imported my forward.fastq.gz, reverse.fastq.gz and merged barcodes.fastq.gz using this command:
qiime tools import
Next I ran qiime demux emp-paired with this command:
qiime demux emp-paired
And that produces this error:
Traceback (most recent call last):
File “/hpc/apps/qiime2-2019.1/install/lib/python3.6/site-packages/q2cli/commands.py”, line 274, in call
results = action(**arguments)
File “</hpc/apps/qiime2-2019.1/install/lib/python3.6/site-packages/decorator.py:decorator-gen-422>”, line 2, in emp_paired
File “/hpc/apps/qiime2-2019.1/install/lib/python3.6/site-packages/qiime2/sdk/action.py”, line 231, in bound_callable
File “/hpc/apps/qiime2-2019.1/install/lib/python3.6/site-packages/qiime2/sdk/action.py”, line 365, in callable_executor
output_views = self._callable(**view_args)
File “/hpc/apps/qiime2-2019.1/install/lib/python3.6/site-packages/q2_demux/_demux.py”, line 331, in emp_paired
for barcode_record, forward_record, reverse_record in seqs:
File “/hpc/apps/qiime2-2019.1/install/lib/python3.6/site-packages/q2_demux/_demux.py”, line 194, in iter
'Barcode header lines do not contain description fields ’
ValueError: Barcode header lines do not contain description fields but sequence header lines do.
Plugin error from demux:
Barcode header lines do not contain description fields but sequence header lines do.
See above for debug info.
At this point, I do not see what the issue is. What could be the problem here?