I am back in using q2-itsxpress with qiime2.2020.11 and I got the flowing error ~$ qiime itsxpress trim-pair-output-unmerged --i-per-sample-sequences demux_sp001.qza --p-region ITS1 --p-taxa F --p-cluster-id 1.0 --o-trimmed demux_sp001_trimmed.qza Plugin error from itsxpress: Command '['bbmerge.s…

You need to update bbtools from within your qiime2 conda environment. It uses a feature only found in more recent versions.

Still got error when installing bbtools on qiime env: conda install -c agbiome bbtools Collecting package metadata (current_repodata.json): done Solving environment: failed with initial frozen solve. Retrying with flexible solve. Solving environment: failed with repodata from current_repodata.js…

Yes, you need a newer version of bbtools. Uninstall the versions you installed with conda and pip then reinstall this version (note the new conda repository and the name bbmap not bbtools). conda install -c bioconda bbmap By the way, what version of Qiime2 are you using?

Hi Dr. Adams I tried your recommendation. I uninstalled the conda and pip versions and then the qiime2. I activated the conda environment and downloaded the itsxpress and the pip q2-itsxpress. and I used the command conda install -c bioconda bbmap however I keep getting an error. bbtools is not sho…

If you have uninstalled it you can try asking for the newest version, sometimes conda will use a cached version otherwise. `Conda install -c bioconda bbmap=38.92`

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