q2-boots and multiple metrics

Hey QIIME 2 team!

I’m trying to use q2-boots for a project where I’m updating my rarefaction. Typically, I calculate a single table and then use it as the basis of all of my diversity calculations. (Computational effeciency and consistency FTW). I just want to check if I can still do this iwth the new q2-boots model. that if I rarefy my table into a collection, run it through q2-diveristy to calculate my alpha and beta, that I can still pool my data without losing the provenance.

Thanks,

Justine

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Update for future users (possible me in 6 months)

In the python API, you can just pass a dictionary of alpha diversity vectors to q2-boots alpha-average. TBD if this is true for beta metrics.

Is there some magic to convert a folder to a collection for the CLI users that I might be missing?

Thanks,

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Yes! Providing the folder should be sufficient, although you are stuck with the order the filsystem gives. If you don't want this, you can create a .order file inside that directory and provide the filenames in the order you want, 1 filename per line.

4 Likes