Recently, I noticed some weird outputs from the q2-ANCOM, which encouraged me to test the ANCOM-2 in R using the original script from the authors of ANCOM. By comparison, I realized that the output from q2-ANCOM may not be reliable but ANCOM-2 is fully reproducible and robust. I happened to communicate off late with the senior author of ANCOM and received the link to download the latest ANCOM-2 R package. I am told that the volcano plot will be added pretty soon to the package. Hope this will help those who are using ANCOM for predicting the differential features.