A couple of super short observations @SoilRotifer @Nicholas_Bokulich as I'm following this tutorial for COI work. The block of code following the following section needs a couple of tiny adjustments:
As it currently is shown:
qiime rescript dereplicate \
--i-sequences silva-138-ssu-nr99-seqs-filt.qza \
--i-taxa silva-138-ssu-nr99-tax.qza \
--i--p-perc-identity 97 \
...
It's just a type with the last line I've shown in the code block above ^^.
Replace --i--p-perc-identity
with --p-perc-identity
like this:
qiime rescript dereplicate \
--i-sequences silva-138-ssu-nr99-seqs-filt.qza \
--i-taxa silva-138-ssu-nr99-tax.qza \
--p-perc-identity 97 \
...
Along the same lines, the expected value in the --p-perc-identity
is now expected to be a value between 0 and 1, instead of an integer from 0 to 100. So in reality, the code I'd need to run would be:
qiime rescript dereplicate \
--i-sequences silva-138-ssu-nr99-seqs-filt.qza \
--i-taxa silva-138-ssu-nr99-tax.qza \
--p-perc-identity 0.97 \
...
Thanks for the great tool - more to come on my end soon with the COI tests.