I am a new Qiime 2 user. When analyzing joined reads according to tutorial (https://docs.qiime2.org/2020.2/tutorials/read-joining/), I met some troubles.
Firstly, the“manifest file”is a tab-seperated (i.e., .tsv) text file as the description in “Importing data” chapter (https://docs.qiime2.org/2020.2/tutorials/importing/). However, in “Alternative methods of read-joining in QIIME 2”, it seems to be comma-separated.
When a .tsv text file was used, an error was reported.
When a .csv text file was used, data can be imported (although it cannot be viewed using qiime metadata tabulate).
Then, I denoised the sequences with Deblur. No error was reported. However, the results of feature table and rep-seqs seemed not right. Each feature was observed in one sample! It is unusual and I don’t know how to deal with it.
It would be great if some help can be provided.
Thank you in advance.