Problem with vizualization of SSU in bar plot

User Support
, , ,
3

Hello colinbrislawn,

I used tutorial by SoilRotifer to processing, filtering and evaluation the SILVA database with Rescript from this forum: https://forum.qiime2.org/t/processing-filtering-and-evaluating-the-silva-database-and-other-reference-sequence-data-with-rescript/15494

I got SILVA data by Hard Mode from this tutorial.

Citations:

  1.  Forster, D., Filker, S., Kochems, R., Breiner, H. W., Cordier, T., Pawlowski, J., & Stoeck, T. (2019). A comparison of different ciliate metabarcode genes as bioindicators for environmental impact assessments of salmon aquaculture. Journal of Eukaryotic Microbiology, 66(2), 294-308.

1391F 5′-GTACACACCGCCCGTC-3′
Euk B 5′-TGATCCTTCTGCAGGTTCACCTAC-3′

  1.  Günther, B., Jourdain, E., Rubincam, L., Karoliussen, R., Cox, S. L., & Arnaud Haond, S. (2022). Feces DNA analyses track the rehabilitation of a free-ranging beluga whale. Scientific Reports, 12(1), 1-7.

SSUF04 GCTTGTCTCAAAGATTAAGCC
SSURmod CCTGCTGCCTTCCTTRGA