What is the exact command you are trying to run?
This error typically is related to unmatching sample ID names between your metadata file and the ones used to import your sequences. For example, see a similar error here. Can you double check to make sure the sample names in your metadata file are matching the ones you used to import your reads exactly? You can start by the samples mentioned in the error. S270, S268 ..etc
Hope that gets things started!
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