Hello,
I am trying to use the dada2 denoise-paired command on version 2017.8 but I keep experiencing the same error. The code I'm using is:
qiime dada2 denoise-paired \
--i-demultiplexed-seqs ~/data/djcabral/qiime2_test/demux.qza \
--o-table ~/data/djcabral/qiime2_test/table.qza \
--o-representative-sequences ~/data/djcabral/qiime2_test/rep-seqs.qza \
--p-trim-left-f 20 \
--p-trim-left-r 20 \
--p-trunc-len-f 240 \
--p-trunc-len-r 180 \
--p-n-threads 0
The command will run for a while (usually over an hour) before giving me the following error:
Plugin error from dada2:
An error was encountered while running DADA2 in R (return code 1),
please inspect stdout and stderr to learn more.
Debug info has been saved to /users/djcabral/data/djcabral/qiime2_test/qiime2-q2cli-err-8is8dwp6.log
I have attached the log file that is generated. It appears that the error occurs during chimera detection but I can't quite figure out the issue or how to fix it. Does anyone have any ideas or suggestions? Thanks in advance!
error.txt (248 Bytes)
qiime2-q2cli-err-8is8dwp6.txt (7.2 KB)