Problem trying to import my data with cassava

[Nicholas_Bokulich]

HI @Natalie,
Your filenames are not Casava format. I'd recommend sticking with the manifest format, especially as you are already asking for support with the manifest format in this topic:

Problem importing my data in qiime 2

Thank you, both. But it is still not working for me. If I use the new format, in .tsv, which looks like this: sample-id forward-absolute-filepath reverse-absolute-filepath CONTROL_S47_L001 /usr/marinaq/Desktop/IRBI/petrels_metab/amplicons_paired/CONTROL_S47_L001_R1_001.16S1F_5’-3’.fq.trimmed.paired.fastq /usr/home/biopatic/querejeta/mydata/amplicons_paired/CONTROL_S47_L001_R2_001.16S2R_5’-3’.fq.trimmed.paired.fastq CV10_S34_L001 /usr/home/biopatic/querejeta/mydata/amplicons_paired/CV1…

It also looks like your files will match the Casava name conventions if you just remove "16S1F_5’-3’.fq.trimmed.paired." from each filename. You can give that a try and see if it imports — but if it fails then you will need to stick with manifest format, since that is the format your data are truly in!