I am trying to start working with QIIME and is seams I am not able to import my data.
I am trying to import data from 1 sample (I thought I should keep it simple in the beginning, but is not.) obtained by MiSeq (PE250). I have one folder with the forward (I guess) and one reverse. They should contain the seq. without primers and barcodes.
Firstly I was using Casava 1.8 paired-end demultiplexed fastq importing option and I got the usual error message:
Missing one or more files for CasavaOneEightSingleLanePerSampleDirFmt: ‘.+_.+_L[0-9][0-9][0-9]_R_001\.fastq\.gz’
I had changed the name of my files accordingly to the QIIME name format and I still got the error message.
So I try to import them vis the Manifest File.
Now I get this error message:
There was a problem importing manifest:
manifest is not a(n) PairedEndFastqManifestPhred33V2 file:
Filepath on line 1 and column “forward-absolute-filepath” could not be found (/Ips-larvae-B/Ips.larvae.B/H000613-1a_01_L001_R1_001.fq.gz) for sample “sample1”.
Can you please help?
This is how my manifest file looks like:
And this is how my reads look like: