I have recently trained the newly released EUKARYOME database in QIIME2 format. More information can be found on the official website (https://eukaryome.org/).
The EUKARYOME database integrates other databases alongside UNITE version 9.0. Based on my experience, it has been more effective in detecting unassigned species that UNITE ver10.0 couldn't assign.
Of course, each has its pros and cons, but I hope you find it useful for your research. I am sharing the classifier file here.
Thank you, and I look forward to hearing about your results!
Thanks for sharing. Could you please inform us about the QIIME2 version you've used to train the classifier? For successful attempts we need to equalize the Q2/sklearn version for testing. Also, have you by any chance created a phylogenetic tree to use for phylogenetic metrics as well to go along with the taxonomic classification?
The version of QIIME I'm using is 2024.2. I haven't been using QIIME for very long, so I haven't constructed a phylogeny tree with a database yet.
However, is it possible that you're referring to something like the sample phylogenetic tree using "qiime phylogeny align-to-tree-mafft-fasttree"?
I'd appreciate it if you could share any related resources.
I have trained this very same database in QIIME2 format, but using their SSU v1.9 files. I've followed the process indicated on their website, in the QIIME2 - integration section.
Here is the link (I also added your file in it, without any changes):
I am training again the ITS classifier as it isn't compatible with my version of sklearn (version 1.4.2 ). Thus, I've removed Soyeon Kim's file from my drive.
I'll update the Google Drive link when the other classifiers will be trained.
All are trained with sk-learn version 1.4.2, and Eukaryome database files v1.9.
Here is a github repository where you can find a script in order to train the classifiers yourself, in case the sklearn version isn't compatible or a new version of the database will be available:
In case of any updates or changes, the readme or comments in the drive will be updated for you to see.