I have recently trained the newly released EUKARYOME database in QIIME2 format. More information can be found on the official website (https://eukaryome.org/).
The EUKARYOME database integrates other databases alongside UNITE version 9.0. Based on my experience, it has been more effective in detecting unassigned species that UNITE ver10.0 couldn't assign.
Of course, each has its pros and cons, but I hope you find it useful for your research. I am sharing the classifier file here.
Thank you, and I look forward to hearing about your results!
Thanks for sharing. Could you please inform us about the QIIME2 version you've used to train the classifier? For successful attempts we need to equalize the Q2/sklearn version for testing. Also, have you by any chance created a phylogenetic tree to use for phylogenetic metrics as well to go along with the taxonomic classification?
The version of QIIME I'm using is 2024.2. I haven't been using QIIME for very long, so I haven't constructed a phylogeny tree with a database yet.
However, is it possible that you're referring to something like the sample phylogenetic tree using "qiime phylogeny align-to-tree-mafft-fasttree"?
I'd appreciate it if you could share any related resources.