When I ran following code
I got the error
What is wrong with it？
The vsearch error should be in that .log file mentioned at the very end.
Can you post that too so we can take a look?
Thank you for your help.
I ran following code to view the contents of the error log.
I got the following error：
That qza file has a fastq file inside of it, and it needs a fasta file instead.
I imagine you are following the ITS express tutorial.
Are you still using qiime2 version 2022.8?
What version of ITS Express are you using?