I am a new-ish QIIME2 user so I apologize for any incorrect terminology use throughout my post. I have tried my best to explain my issue and provide necessary information, but please let me know if there is anything I am missing.
I have been using QIIME2-2018.4 to follow the "Moving Pictures" tutorial with my own data (5 separate datasets) and everything has gone well! However, I am now trying to merge all 5 datasets together. I was able to successfully run the following commands to merge the sequences and FeatureTables from all 5 datasets:
qiime feature-table merge-seqs
qiime feature-table merge
However, when I try and run these commands to create a visualization of the merged FeatureTable, the bold part works, but the italicized part brings up an error:
qiime feature-table summarize _
_ --i-table table-dada2-USDA-allPATH.qza _
_ --o-visualization table-dada2-USDA-allPATH.qzv _
_ --m-sample-metadata-file Merged_Mapping_Files_8_29_2018.tsv
qiime feature-table tabulate-seqs
The error is as follows:
From this, I think it's safe to assume that the error is in reference to my broccoli data, as that dataset has exactly 72 sample IDs. However, in my mapping file they have the following naming convention: B.001-B.072. So I went in and changed all the IDs to match what was stated in the error (i.e. 1-72) and tried to run the command again. However, when I did this, I got the same error as listed above but stating that the IDs B.001-B.072 were not listed in the metadata.
Here is a visualization of what some of my metadata looks like:
I'm not really sure how to troubleshoot from here and would appreciate any suggestions and guidance! Thank you in advance!