I am having problem to generate alpha diversity. Until this step everything is ok, but when I do this command: qiime diversity alpha-group-significance
I receive this message:
Plugin error from diversity:
Metadata does not contain any columns that satisfy this visualizer’s requirements. There must be at least one metadata column that contains categorical data, isn’t empty, doesn’t consist of unique values, and doesn’t consist of exactly one value.
Debug info has been saved to /var/folders/ll/_2t9tfrd5rd5wx01ksb41gs00000gn/T/qiime2-q2cli-err-qnmq1cn3.log
I dont know what is happening with my metadata. I did the validation on https://keemei.qiime2.org/ and it said that was everything alright.
my metadata (.txt) is annexed below