Hi all
1.time qiime feature-table summarize --i-table table.qza --m-sample-metadata-file metadata.txt --o-visualization table.qzv
2.time qiime feature-table tabulate-seqs --i-data repseqs.qza --o-visualization repseqs.qzv
This prompts me when I successfully run the first line of code"time qiime feature-table summarize --i-table table.qza --m-sample-metadata-file metadata.txt --o-visualization table.qzv"
real 13m36.151s
user 13m34.0385
SVS 0m2.0945
Then I ran the second line of code,the following error message appears
Plugin error from demux:
Value must be a nonnegative integer or None
Debug info has been saved to /tmp/qiime2-q2cli-err-6lsl970n.log
Where is the problem? Is there a problem with the parameter Settings in demux? Or is there a problem with the raw data? How can I solve this problem?Hope you can help me.