Hi all
I got this error in demultiplexing my data by using following command:
= qiime tools import **
–type EMPPairedEndSequences **
–input-path emp-paired-end-sequences **
–output-path emp-paired-end-sequences.qza
Demultiplexing
qiime demux emp-paired **
–m-barcodes-file sample-metadata.tsv **
–m-barcodes-column barcode-sequence **
–p-rev-comp-mapping-barcodes **
–i-seqs emp-paired-end-sequences.qza **
–o-per-sample-sequences demux.qza **
–o-error-correction-details demux-details.qza
Plugin error from demux: Plugin error from demux: Golay decoding requires 12nt barcodes. The barcode attempting to be decoded (GAGTACAG) is of length 8nt.
i tried --p-no-golay-error-correction** in command but not working
qiime demux emp-paired **
–m-barcodes-file sample-metadata.tsv **
–m-barcodes-column barcode-sequence **
–p-rev-comp-mapping-barcodes **
–p-no-golay-error-correction\
–i-seqs emp-paired-end-sequences.qza **
–o-per-sample-sequences demux.qza **
–o-error-correction-details demux-details.qza
got following error again:
(1/1?) no such option: --p-no-golay-error-correction–i-seqs (Possible
options: --p-golay-error-correction, --p-no-golay-error-correction)