I have been trying to run dada2 denoise -paired on the same set of data that I have worked before. I accidentally deleted all my files from my server and have been trying to rerun the exact same codes using the exact same parameters on the same dataset. Each time I try running the dada2 it gives back the following error:
Plugin error from dada2:
No reads passed the filter. trunc_len_f (160) or trunc_len_r (105) may be individually longer than read lengths, or trunc_len_f + trunc_len_r may be shorter than the length of the amplicon + 12 nucleotides (the length of the overlap). Alternatively, other arguments (such as max_ee or trunc_q) may be preventing reads from passing the filter.
I have looked in the forum for related queries and saw users having the same issue but it had a lot to do with their overlap length. I have been working on the same dataset and did not have a problem a month ago (using the same parameters). I'm including the codes that I ran and the debug info. Can someone please help me with this or explain to me why this is happening?
qiime dada2 denoise-paired